Although HRMA heterozygote detection is excellent with a rate of 100%17, successful discrimination between homozygous variants that melt in a single domain depends mostly on their Tm with an average detection rate of 75% from 6 prior studies using constitutional human variants8,17,18. The ability to use absolute temperatures differences for genotyping depends on the temperature precision of the instrument19 and the consistency (ionic strength) of compared samples20. Interestingly, best homozygous variants detection rates of 93% and 96.5% were reported for human BRCA1 and mitochondrial DNA21,22.
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There must be a way to get into the movies for less, you’d think. In fact, there are several ways. We’re not going to go so far as to suggest you smuggle in microwave popcorn and canned soda in a big purse shame on us for even thinking such a thing! but here are some tips on saving at the cinema:.
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