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Although HRMA heterozygote detectio

Although HRMA heterozygote detection is excellent with a rate of 100%17, successful discrimination between homozygous variants that melt in a single domain depends mostly on their Tm with an average detection rate of 75% from 6 prior studies using constitutional human variants8,17,18. The ability to use absolute temperatures differences for genotyping depends on the temperature precision of the instrument19 and the consistency (ionic strength) of compared samples20. Interestingly, best homozygous variants detection rates of 93% and 96.5% were reported for human BRCA1 and mitochondrial DNA21,22.

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